Goals
Improve wet lab skills through work in neurodegeneration; pursue a doctoral degree to expand knowledge and skills for developing therapies for neurodegenerative disorders; improve teaching and quality of care with a focus on cost-effectiveness of diagnostic and therapeutic approaches.
pursue a doctoral degree in human neurodevelopmental genetics to develop cell/gene-based therapies in neurology;
Improve clinical research skills to become a better physician scientist; pursue a doctoral degree in human neurodevelopmental genetics to develop cell/gene-based therapies in neurology
phd in neurodevelopment, translating findings to improve teaching and quality of care, with a focus on optimizing cost ratios of diagnostics and therapeutics.
Key Skills
- Clinical care: assessment and ongoing management of post-operative and acutely unwell patients; monitoring and escalation; safe prescribing and documentation; patient and family communication.
- Procedures / sampling: wound care and suturing; specimen collection and handling (blood, CSF, bone marrow, tissue) with attention to infection control and sample integrity.
- Stem cell & neurodevelopment wet lab: iPSC-derived human cortical progenitors; lentiviral transduction; CRISPR perturbation workflows; immunocytochemistry / immunohistochemistry; confocal/fluorescence microscopy; flow cytometry (FACS).
- Bioinformatics & data analysis: whole-genome CRISPR knockout screen analysis; differential analysis and hit calling; RNA sequencing and single-cell RNA sequencing workflows; R-based data wrangling and visualisation; Linux command-line work.
- Research delivery: experimental design and troubleshooting; standard operating procedures drafting; quality control of equipment/processes; procurement and lab operations; mentoring and supervision of trainees/students.
- Communication & leadership: led journal club discussions, organised workshops/meetings; cross-team coordination; clear scientific writing and presentation.
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Employment and Academics
2019 – 2021 — Masters in Neuroscience
University College London, UK — Prof. Rick Livesey, Great Ormond Street Hospital (GOSH), Institute of Child Health (ICH), Zayed Centre for Research into Rare Disease in Children.
- Investigated genes involved in neuronal progenitor expansion (MED12, CTXN1) in iPSC-derived human cortical progenitors.
- Analyzed whole-genome CRISPR KO screens to identify genes in human neurodevelopment.
- Coursework: Receptors and Synaptic Signalling; Developmental Neuroscience; Systems and Circuits Neuroscience; Neurobiology of Degeneration and Repair.
2020 Jun – Dec — Duty Doctor
Aditya Hospitals, Hanamkonda, Warangal. Interrupted Masters to support COVID-19 response.
2018 – 2019 — Voluntary Research Assistant, Student Recruiter & Mentor
Prof. G. Madhava Reddy Research Foundation (GMRRF), Uppal, Hyderabad.
- Recruited, oriented, and trained staff.
- Prepared publication on PIH genetics (ACE1 I/D polymorphism) with State hospital collaboration; developed VEGF polymorphism project and pilot on seasonal PIH prevalence.
- Publication preparation on Genetics of Pregnancy Induced Hypertension.......
- recruited, reviewed applications, and interviewed student candidates.
- hardware; replacements; repaired an ultra-centrifuge certified “dead”.
- ensured quality, safety, and security compliance.
- Mentored seven undergraduate/graduate students; organized workshops; reviewed applications and conducted interviews for student recruitment.
- Maintained lab, QC of equipment; fabricated a 128-lane electrophoresis rig; repaired an ultra-centrifuge certified “dead”.
- Drafted SOPs; implemented new methods, instrumentation, and procedures; procured and negotiated ordering of consumables/equipment/services.
- Led project development, experiment execution, data analysis, and coordinated staff/admin; mentored students in research projects and report writing.
2018 Sep — Training
Cell biology and cell culture techniques, Centre for Cellular & Molecular Biology (CCMB), Hyderabad.
2017 Jul – Nov — Training
Biotechnology, Prof. G.M. Reddy Research Foundation (GMRRF), Uppal, Hyderabad.
2017 May – Jun — Training
Molecular genetics (Huntington disease), GeneTech Labs, Hyderabad.
2015 – 2017 — Duty Doctor
Seven Hills Hospital, Hanamkonda, Warangal.
- Managed post-operative and critically ill patients.
- Assisted minor clinical procedures.
2014 – 2015 — Internship (50–80 hrs/week)
Kakatiya Medical College affiliated hospitals, Warangal.
- Performed several minor and assisted major surgical/medical procedures, including labour conduction.
- Data collection/analysis for thesis “Surgical Wound Gaping”.
- Assisted theses on Diabetic Ulcer scale and Pancreatic Necrosis.
- Rotations: CKMH (Maternity), MGMH Blood Bank, CHC (Wardhannapet), UHC (Ursu), Warangal.
2009 – 2014 — MBBS
Kakatiya Medical College, Warangal (Dr. NTRUHS).
- Distinctions in Pharmacology, Gynaecology & Obstetrics.
- Extensive coursework in Anatomy (cadaver dissections, histology), Neuroanatomy, Genetics, Biochemistry (clinical biomolecule quantification), Molecular Biology, Physiology (including Haematology), Microbiology (pathogen identification and microscopy), Parasitology, Pathology (gross/histological), General and Clinical Pharmacology (identification of pharmacological compounds), Immunology, Toxicology, SPM/community medicine with fieldwork and outreach.
- Learning patient care and treatment modalities; clinical exposure across Ophthalmology, ENT, General Medicine, General Surgery (performed minor surgeries; assisted major procedures), Orthopaedics, Paediatrics, Obstetrics & Gynaecology (labour conduction; assisted C-sections), Psychiatry, Dermatology, Radiology; led group discussions and mentored juniors on clinical skills, procedures, and differential diagnosis.
- Graduated with 67.4%; among top of 2009 cohort.
2007 – 2009 — Intermediate Education
Nava Vignana Bharathi Junior College, Warangal. Majors: Physics, Chemistry, Botany, Zoology; Sanskrit as second language. Best outgoing; 97.8% in majors, 94.5% overall.
Up to 2007 — Primary & Secondary
Learners’ Land High School, Warangal. Best outgoing student; 86.0%.
Qualifying Exams
- GRE: 314/340 (155 Verbal, 159 Quantitative, AWA 3.0).
- IELTS: R-8.5, L-8.5, S-7, W-6.5.
Technical Skills
- Lentiviral CRISPR; iPSC-derived human neuronal progenitors; primary culture (basics); MTT, invasion, FACS, angiogenesis, migration, efflux, and cell counts.
- CRISPR KO screen analysis: MAGeCK, edgeR, BAGEL2, CB2; guide efficiency prediction; differential gene expression; large dataset handling (whole-genome screens).
- Confocal/fluorescence imaging; image analysis; ICC/IHC; staining; specimen collection/handling.
- Molecular biology: DNA extraction (blood/tissue/plants), primer design, PCR, electrophoresis, HPLC.
- Microbiology: culture, cloning, plasmid isolation, transformation, identification.
- Dissection & surgical skills; sampling (CSF, bone marrow, tissue, blood); biomolecule identification; staining.
- Extensive lab work in Anatomy, Biochemistry, Physiology, Histology, Microbiology, Pathology, Haematology, Immunology; SPM fieldwork.
- Intermediate R scripting; large dataset handling (whole-genome KO screens); data wrangling/EDA; visualization; Linux virtualization/terminal.
- Basics in Python; NGS data analysis; bedtools; Galaxy pipelines; bioinformatics; biostatistics; SPSS; Mutation Surveyor; Chromas.
- NGS data analysis: RNA-Seq (trimming, HISAT2, HTSeq), scRNA-seq analysis, CRISPR screen analysis, bedtools for genomic annotation, Galaxy pipelines.
- Programming in C++; built console app for uninterrupted network connectivity and adaptive display brightness.
- Built/configured server-grade compute machines for hospital data management/workflows.
Digital & Automation Skills
- Rapid prototyping of small tools for research/workflow automation (Python scripting and command-line workflows).
- Structured documentation and interactive resources (HTML/CSS/JavaScript) for organising study/research material and contact directories.
- Comfortable working across Windows and Linux environments; reproducible analysis setups and large-file/data handling.
- Public sharing of technical notes/tutorials (YouTube) and code/workflows (GitHub).
- Smart-home/voice-assistant tinkering and community engagement (Home Assistant Community forum).
Achievements
- Rank 204 in EAMCET (~60,000 candidates).
- Rank 1763 in AIIMS Entrance Exam (~30,000 candidates).
Presentations, Seminars & Organizational Skills
- 17 Jan 2019: Poster on functional analysis of genes regulating human cortical progenitor dynamics, UCL.
- 2019–2021: Led presentations/discussions in Neuroscience Journal Club, UCL.
- Nov 2014: Poster prize, “Stem Cells - Recent developments”, awarded by Ch. Mohan Rao (CCMB) at Uthkarsha, Kakatiya Medical College.
- 30–31 Aug 2014: Communications lead for “Kakatiya Surge” CME Seminar in General Surgery, Warangal.
- 2012: Managed registrations for State Pathology Conference on “Small Round Cell Tumours”, Kakatiya Medical College, Warangal.
- Organized and presented seminars (“Pathogenesis of Aortic Aneurysm”, “Metabolic change after Injury”), theses, case presentations across Kakatiya Medical College hospitals.